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KMID : 0848120050300040135
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International Journal of Oral Biology
2005 Volume.30 No. 4 p.135 ~ p.141
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Effect of TGF- β1 on Osteoclast Differentiation
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Park Su-Jin
Ko Jea-Seung
Kim Hyun-Man
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Abstract
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Although it has been known that TGF-β1 acts as a crucial cofactor in osteoclast differentiation, its mode of action is still unclear. In the present study, we studied the effect of TGF-β1 on the differentiation of osteoclast depending on the developmental stages. Murine bone marrow cells were induced to differentiate into mature osteoclasts in the presence of receptor activator of NF-κB ligand (RANKL) and macrophage colony stimulating factor (M-CSF). In the early stage of the differentiation TRAP(-) mononuclear precursor cells were obtained from nonadherent M-CSF dependent bone marrow cells, which further differentiated into mature osteoclasts. TGF-β1 stimulated osteoclast differentiation, which was stronger when cells were stimulated by TGF-β1 in the early stage than the later differentiation. TGF-β1 increased the expression of RANK and synergistically stimulated RANKL-induced activation of NF-κB MAP kinase in TRAP(-) mononuclear precursor cells. These results suggest that activation of osteoclast differentiation by TGF-β1 may be ascribed to the both increased expression and activation of RANK in the osteoclast differentiation, especially in the early stage of differentiation
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KEYWORD
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Osteoclast, TGF-β, differentiation, bone
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